Scaling up DNA origami lattice assembly

Y. Xin, B. Shen, M.A. Kostiainen, G. Grundmeier, M. Castro, V. Linko, A. Keller

Chemistry - A European Journal Vol. 27, nº. 33, pp. 8564 - 8571

Summary:

The surface‐assisted hierarchical assembly of DNA origami nanostructures is a promising route to fabricate regular nanoscale lattices. In this work, the scalability of this approach is explored and the formation of a homogeneous polycrystalline DNA origami lattice at the mica‐electrolyte interface over a total surface area of 18.75 cm² is demonstrated. The topological analysis of more than 50 individual AFM images recorded at random locations over the sample surface showed only minuscule and random variations in the quality and order of the assembled lattice. The analysis of more than 450 fluorescence microscopy images of a quantum dot‐decorated DNA origami lattice further revealed a very homogeneous surface coverage over cm² areas with only minor boundary effects at the substrate edges. At total DNA costs of € 0.12 per cm², this large‐scale nanopatterning technique holds great promise for the fabrication of functional surfaces.

Spanish layman's summary:

El origami de ADN se une a la nanotecnología para producir superficies funcionales a gran escala con un costo total de ADN de 0,12€ por cm2.

English layman's summary:

DNA origami meets nanotechnology to produce large-scale functional surfaces under total DNA costs of € 0.12 per cm2.

JCR Impact Factor and WoS quartile: 5,020 - Q2 (2021); 3,900 - Q2 (2023)

DOI reference: https://doi.org/10.1002/chem.202100784

Published on paper: June 2021.

Published on-line: March 2021.

Citation:
Y. Xin, B. Shen, M.A. Kostiainen, G. Grundmeier, M. Castro, V. Linko, A. Keller, Scaling up DNA origami lattice assembly. Chemistry - A European Journal. Vol. 27, nº. 33, pp. 8564 - 8571, June 2021. [Online: March 2021]