Scaling up DNA origami lattice assembly

Y. Xin, B. Shen, M.A. Kostiainen, G. Grundmeier, M. Castro, V. Linko, A. Keller

Chemistry - A European Journal Vol. 27, nº. 33, pp. 8564 - 8571

Resumen:

The surface‐assisted hierarchical assembly of DNA origami nanostructures is a promising route to fabricate regular nanoscale lattices. In this work, the scalability of this approach is explored and the formation of a homogeneous polycrystalline DNA origami lattice at the mica‐electrolyte interface over a total surface area of 18.75 cm² is demonstrated. The topological analysis of more than 50 individual AFM images recorded at random locations over the sample surface showed only minuscule and random variations in the quality and order of the assembled lattice. The analysis of more than 450 fluorescence microscopy images of a quantum dot‐decorated DNA origami lattice further revealed a very homogeneous surface coverage over cm² areas with only minor boundary effects at the substrate edges. At total DNA costs of € 0.12 per cm², this large‐scale nanopatterning technique holds great promise for the fabrication of functional surfaces.

Resumen divulgativo:

El origami de ADN se une a la nanotecnología para producir superficies funcionales a gran escala con un costo total de ADN de 0,12€ por cm2.

Índice de impacto JCR y cuartil WoS: 5,020 - Q2 (2021); 3,900 - Q2 (2023)

Referencia DOI: https://doi.org/10.1002/chem.202100784

Publicado en papel: Junio 2021.

Publicado on-line: Marzo 2021.

Cita:
Y. Xin, B. Shen, M.A. Kostiainen, G. Grundmeier, M. Castro, V. Linko, A. Keller, Scaling up DNA origami lattice assembly. Chemistry - A European Journal. Vol. 27, nº. 33, pp. 8564 - 8571, Junio 2021. [Online: Marzo 2021]